B-Ionic Calcium Buffer System does not contain phosphates or nitrates. In addition to supplying highly concentrated calcium and carbonate alkalinity required for calcification, B-Ionic Calcium Buffer System also provides all other important major, minor, and trace elements in the proper ratios to duplicate the composition of natural seawater. As a result, B-Ionic Calcium Buffer System will help restore inorganic ions lost from protein skimming and help maintain the ionic balance of the aquarium water. Starting daily dosage is 1 ml per 4 gallons of aquarium capacity. Gallon-sized bottles are concentrated so they will need to be diluted with RO/DI (or distilled) water before use.